In today’s competitive genomics landscape, RNA sequencing will help you quickly obtain your desired results. Our xGen RNA sequencing product offerings cover a wide range of diverse inputs while maintaining predictable outputs, efficiencies, and coverage metrics.
xGen™ NGS—made to differentiate.
RNA sequencing (RNA-seq) is a next generation sequencing method for analyzing the transcriptome of cells and assessing gene expression variation. The xGen RNA Library Prep Kit and the xGen Broad-Range RNA Library Prep Kit are fast transcriptomics research workflow solutions with comprehensive transcript coverage and NGS data quality.
The workflow used in these kits employs Adaptase™ technology which adds a single-stranded R2 Stubby Adapter onto the 3’ end of the 1st strand cDNA product. This technology does not require 2nd strand cDNA synthesis and degradation and results in stranded RNA libraries. While the xGen Broad-Range RNA Library Prep Kit supports a wider range of total RNA inputs (from 10 ng to 1 µg) than the xGen RNA Library Prep Kit (from 100 ng to 1 µg), the latter offers a faster workflow with fewer steps. This RNA-seq workflow is compatible with upstream mRNA enrichment and rRNA depletion modules. It is easily combined with a hybridization capture workflow for a targeted mRNA capture sequencing study.
For high-throughput labs, the 96-reaction size group of products includes the xGen Normalase Module, a proprietary enzymatic library normalization kit for multiplexing. The method eliminates the need for individual sample quantification followed by manual equimolar pooling. Instead, the fragments are indexed by PCR using the included plate of xGen Normalase UDI Primer Pairs followed by the xGen Normalase Module. Final normalized library pools are then ready for Illumina-based multiplex sequencing.
xGen RNA Library Prep Kit (everyday samples)
xGen Broad-Range RNA Library Prep Kit (low-quality or low-input samples)
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.