Processing
SciTools™ Web Tools
These software applications determine the properties of any oligo sequence entered, as well as facilitate the intelligent design of assay conditions, all at the click of a button. The suite is easy to use and makes it simple to add each optimized oligonucleotide to an ongoing order list ready for purchase.
Oligo design & handling
- OligoAnalyzer™ Tool
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
- UNAFold Tool
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
- Dilution Calculator
Find the volume needed to dilute your oligo stock to a lower concentration
- Resuspension Calculator
Find the volume needed to resuspend a dry oligo to a desired concentration
qPCR assay design
- PrimeTime™ Predesigned qPCR sequences
Selects qPCR primers and probe sequences for human, mouse, and rat gene expression assays based on up-to-date sequence data
- PrimerQuest™ Tool
Allows highly customized designs for PCR primers and qPCR assays
- RealTime PCR Tool
Selects custom qPCR assays for any species
Genotyping
- rhAmp™ Genotyping Assay Design Tool
Choose predesigned rhAmp SNP Assays or design assays for SNPs not currently included in the predesigned assay library
CRISPR genome editing
- Alt-R™ Predesigned Cas9 crRNA Selection Tool
Selects guaranteed crRNAs targeting human, mouse, rat, zebrafish, or nematode genes
- Alt-R Custom Cas9 crRNA Design Tool
Designs custom crRNAs to sequences from any species
- Alt-R CRISPR HDR Design Tool
A complete solution for industry-leading HDR rates based on extensive wet bench testing and customer validation
- CRISPR-Cas9 crRNA design checker
Assesses on- and off-targeting potential of user-designed protospacer sequences
- rhAmpSeq™ CRISPR Analysis Tool
Flexible, cloud-based interrogation of CRISPR-mediated, double-strand break repair
Gene regulation & RNAi
- Predesigned DsiRNA Selection Tool
Selects DsiRNAs and TriFECTa® RNAi Kits for your human, rat, or mouse targets
- RNAi Design Tool
Generates DsiRNAs for any sequence from any species
Genes & gene fragments
- Codon Optimization Tool
Automates codon optimization of a gene to the species of your choice
- Genes & gene fragments order status
Track your order (Genes & gene fragments only)
Next generation sequencing
- Custom Adapter Configurator Tool
Guides you through choice of library prep method, configuration, indexing strategy, delivery format, and other options. Order directly online.
- Library Concentration Conversion Calculator
Please use the following format when citing SciTools:
PrimerQuest™ program, IDT, Coralville, Iowa, USA. Accessed 12 December, 2018. https://www.idtdna.com/SciTools.
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.
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