Available in convenient sizes:
|Scale (pmol/oligo)||Number of oligos per pool||Oligo length (bases)|
|1||100 to 20,000||40 to 350|
|10||10 to 2000||40 to 350|
|50||2 to 384||40 to 350|
oPools Oligo Pools are single-stranded DNA sequences that are used for CRISPR library construction, primer pools for multiplex PCR, gene construction, data storage, and FISH analysis.
Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramer™ DNA Oligos. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, oPools DNA Oligos use a unique solid support specifically optimized to synthesize high-quality oligos up to 350 bases in length.
|Scale (number of oligos)|
|Mixed bases ||N = A, C, G, and T|
K = G and T
• Limited to 10 mixed bases per oligo
|QC and quantification||None|
|Shipping container||2 mL tube|
Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.6% (IDT oligos, n = 34,627) and 99.4% (other supplier, n = 77 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.
Figure 2. Proprietary DNA synthesis results in an even yield distribution of oPools Oligo Pools. This even yield distribution is shown here as a function of % difference from the mean. The standard deviation observed across ½ million sequences is less than 23% of the mean, demonstrating a high level of uniform sequence representation.
Dropout rate refers to the likelihood that any individual sequence is not present in the final pool. This rate can vary by length and sequence complexity. The average dropout rate for oPools oligos across all length ranges was 0.8% based on a sample size of over 900,000 oligos.