Processing
Genes & gene fragments
Understanding the importance of 3’ UTR mutations in cancer research
Citation summary: While mostly unexplored and given significantly less attention than coding sequence (CDS) and 5’ untranslated regions (UTR), 3’ UTR somatic mutations affect mRNA stability and translation, making them important subjects to study for cancer researchers to better understand cancer progression. This DECODED summarizes the efforts of researchers from the University of Washington and Fred Hutchinson Cancer Center to characterize 3’ UTR somatic mutations.
- Understanding the importance of 3’ UTR mutations in cancer research
- Luciferase, an alternative to fluorescence
- The lac operon, explained
- Troubleshooting your site-directed mutagenesis by PCR
- Gateway™ cloning: Definitions, tips, and tricks
- How DNA ligation works
- Primer design and other tools you should know about
- Using gBlocks™ HiFi Gene Fragments to explore novel pathways of antibiotic resistance
- Targeting antibiotic resistant microbial infections with synthetic biology
- Antigen-specific B cell enrichment for faster, more efficient antibody discovery
- The molecular characterization of new protein structures using eBlocks™ Gene Fragments
- Site-directed mutagenesis—Improvements to established methods
- Increase genetic stability with synthetic biology
- Characterization of antitumor CD8+ T cells in melanoma to optimize immune response
- Codon optimization tool makes synthetic gene design easy
- gBlocks™ Gene Fragments used in an antibody discovery workflow to test the efficiency of tumor-reactive antibodies
- Systems and synthetic biology: constructing smart and programmable microbes to address global problems
- CRISPR GUARD: A short gRNA-based system that facilitates precise genome edits
- Tips for designing spike-in controls for next generation sequencing analysis
- Tips for working with IDT Gene Fragments
- Cloning strategies: Blunt-end cloning
- Cloning strategies: Cohesive end cloning
- Site-directed mutagenesis methods
- Seamless assembly and cloning
