- Recommended products—curated for whole exome sequencing
- A tested solution—takes you from sample to sequencer
- Flexible sizing—available for 16 reactions or 96 reactions
- Enzymatic fragmentation—no need for specialized equipment for library preparation
- Individually synthesized probes—quality-controlled probes, manufactured to ISO standards, with deep, uniform coverage
- Quality and consistency—high-quality performance metrics, displayed consistently across lots
- Automation friendly—easy workflow that can be automated for high-throughput applications
Whole genome sequencing (WES) is a targeted next generation sequencing (NGS) approach that uses modified oligonucleotide probes to “capture” and enrich the protein coding regions
(exons) in a genome. Focusing only on protein-coding exons can lower the cost and time of sequencing, as exons make up approximately 1% of the genome but contain 85% of the variants that are associated with disease . WES
is a practical application for various fields of research such as populations genetics and oncology research. It is also a feasible option for population genetics and discovery science, or data mining, when searching for associations, or linking genes
to phenotypes . The information gained from WES can provide researchers insights into germline and somatic variants within coding regions of genes.
xGen whole exome sequencing (WES) solution
For WES, samples can be prepared using multiple library preparation methods, the xGen whole exome sequencing solution employs the xGen DNA Library Prep EZ which uses enzymatic fragmentation to shear high-quality DNA samples. This kit uses ligation-based library prep, it includes enzymes for
fragmentation, end-repair, and A-tailing, as well as a ligase for the addition of the xGen Stubby Adapters (included).
The xGen WES solution also calls for premixed xGen UDI Primer Pairs for indexing by PCR, which generates NGS libraries that can be directly used for hybridization capture with the xGen Exome Hyb Panel v2. This panel consists of 415,115 individually synthesized, 5’ biotinylated, oligonucleotide probes. Each probe is quality controlled
and combined equimolarly to help to ensure ideal uniformity of enrichment from each panel. In an internal study, when compared to panels from other vendors, the xGen Exome Hyb Panel v2 had the highest on-target rate and mean coverage, higher depth
coverage across more exons, and a more flexible workflow. Please refer to the application note, The xGen Exome Hyb Panel v2 - human exome sequencing with consistent coverage, for more information.
The xGen whole exome sequencing solution also includes xGen Hybridization Capture Core Reagents such as the xGen Hybridization and Wash Kit, xGen Universal Blockers TS, and xGen Library Amplification Primer Mix.
These reagents, when paired with the xGen Hyb Panel v2, help to provide a complete, high-quality, whole exome sequencing workflow.