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Tom Aune

Tom Aune received a Bachelor of Science from Rhodes College and a Doctor of Philosophy from University of Tennessee Health Sciences Center. He currently is a Full Professor of Medicine at Vanderbilt University Medical Center. 

Collaborator content

A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates

Since the start of molecular biology, plasmid-based cloning has been an essential component of both basic and medical research. A limitation is that plasmid-based cloning is a complicated, multistep process.

This publication presents a new method preparing synthetic genes and mature mRNA that can be efficiently introduced into cells and translated into functional proteins without the need for plasmid-based cloning, other RNA such as long noncoding RNA, can also be prepared using this method.

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